| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Milho e Sorgo. |
|
Data corrente: |
13/03/2018 |
|
Data da última atualização: |
09/05/2018 |
|
Autoria: |
PEREIRA, A. E.; CARNEIRO, N. P.; SIEGFRIED, B. D. |
|
Afiliação: |
Adriano E. Pereira, University of Nebraska; NEWTON PORTILHO CARNEIRO, CNPMS; Blair D. Siegfried, University of Florida. |
|
Título: |
Comparative susceptibility of southern and western corn rootworm adults and larvae to vATPase-A and Snf7 dsRNAs. |
|
Ano de publicação: |
2016 |
|
Fonte/Imprenta: |
Journal of RNAi Gene Silencing, v. 12, p. 528-535, 2016. |
|
Idioma: |
Inglês |
|
Conteúdo: |
Corn rootworms (CRW) are the most important corn pests in the U.S. Corn Belt. Damage is caused by larval feeding on the plant roots. RNAi has been studied in CRW and has proven to be effective for the management of this insect with effects on both larval and adult stages. The objective of this study was to determine comparative susceptibility of adult and larva of Southern Corn Rootworm (SCR) and Western Corn Rootworm (WCR) to two different lethal RNAi target genes (vacuolar (v)-ATPase-A and Snf7). Adults and larvae were exposed to dsRNAs overlaid on artificial diet five times every other day. WCR larval susceptibility to vATPase-A was approximately 4-fold more tolerant than SCR, but were similar for Snf7 (2.6 ng/cm2 for both species). For adults, LC50s were generally higher for vATPase-A dsRNA relative to larvae with a 20-fold higher LC50 for WCR (SCR=33.3 ng/cm2; WCR=657.3 ng/cm2) relative to Snf7 dsRNAs (SCR=13.2 ng/cm2; WCR=60.2 ng/cm2). Gene silencing was also evaluated in adults and larvae exposed to the LC50 dsRNAs, and gene knockdown ranged from 48% up to 83% in adults fed for eight days in dsRNA, and from 55% to 89% in larvae fed for 24, 48 and 72 h, compared to control treatments. This is the first study to report LC50 values for dsRNA in WCR and SCR adults. The results indicate that both larvae and adults of both species were affected by vATPase-A and Snf7 dsRNAs suggesting that RNAi for the management of CRW should be considered for both stages. |
|
Thesagro: |
Gene; Genética; Larva; Praga de planta; Raiz. |
|
Categoria do assunto: |
O Insetos e Entomologia |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/173852/1/Comparative-susceptibility.pdf
|
|
Marc: |
LEADER 02084naa a2200205 a 4500
001 2089001
005 2018-05-09
008 2016 bl uuuu u00u1 u #d
100 1 $aPEREIRA, A. E.
245 $aComparative susceptibility of southern and western corn rootworm adults and larvae to vATPase-A and Snf7 dsRNAs.$h[electronic resource]
260 $c2016
520 $aCorn rootworms (CRW) are the most important corn pests in the U.S. Corn Belt. Damage is caused by larval feeding on the plant roots. RNAi has been studied in CRW and has proven to be effective for the management of this insect with effects on both larval and adult stages. The objective of this study was to determine comparative susceptibility of adult and larva of Southern Corn Rootworm (SCR) and Western Corn Rootworm (WCR) to two different lethal RNAi target genes (vacuolar (v)-ATPase-A and Snf7). Adults and larvae were exposed to dsRNAs overlaid on artificial diet five times every other day. WCR larval susceptibility to vATPase-A was approximately 4-fold more tolerant than SCR, but were similar for Snf7 (2.6 ng/cm2 for both species). For adults, LC50s were generally higher for vATPase-A dsRNA relative to larvae with a 20-fold higher LC50 for WCR (SCR=33.3 ng/cm2; WCR=657.3 ng/cm2) relative to Snf7 dsRNAs (SCR=13.2 ng/cm2; WCR=60.2 ng/cm2). Gene silencing was also evaluated in adults and larvae exposed to the LC50 dsRNAs, and gene knockdown ranged from 48% up to 83% in adults fed for eight days in dsRNA, and from 55% to 89% in larvae fed for 24, 48 and 72 h, compared to control treatments. This is the first study to report LC50 values for dsRNA in WCR and SCR adults. The results indicate that both larvae and adults of both species were affected by vATPase-A and Snf7 dsRNAs suggesting that RNAi for the management of CRW should be considered for both stages.
650 $aGene
650 $aGenética
650 $aLarva
650 $aPraga de planta
650 $aRaiz
700 1 $aCARNEIRO, N. P.
700 1 $aSIEGFRIED, B. D.
773 $tJournal of RNAi Gene Silencing$gv. 12, p. 528-535, 2016.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Milho e Sorgo. |
|
Data corrente: |
17/04/2024 |
|
Data da última atualização: |
23/04/2024 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
A - 4 |
|
Autoria: |
VIANA, J. H. M.; COELHO, A. M.; THOMAZINI, A.; CARVALHO, M. P. F. de; FRANÇA, A. C. F.; CLEMENTE, E. de P. |
|
Afiliação: |
JOAO HERBERT MOREIRA VIANA, CNPMS; ANTONIO MARCOS COELHO, CNPMS; ANDRÉ THOMAZINI, UNIVERSIDADE FEDERAL DE SÃO JOÃO DEL-REI; MATHEUS PAULA FIALHO DE CARVALHO, UNIVERSIDADE FEDERAL DE SÃO JOÃO DEL-REI; ANA CAROLINA FERREIRA FRANÇA, UNIVERSIDADE FEDERAL DE SÃO JOÃO DEL-REI; ELIANE DE PAULA CLEMENTE ALMEIDA, CNPMS. |
|
Título: |
Dry matter production and nutrient extraction by three successive crops fertilized with serpentinite rock as a soil remineralizer. |
|
Ano de publicação: |
2024 |
|
Fonte/Imprenta: |
Observatorio de La Economia Latinoamerica, v. 22, n. 4, p. 1-17, 2024. |
|
DOI: |
https://doi.org/10.55905/oelv22n4-068 |
|
Idioma: |
Inglês |
|
Conteúdo: |
The incorporation of new sources of fertilizers is important to of the food production security. The use of rock powders still needs investigation to address their agronomic efficiency and the safety for the users and for the consumers. This work aimed to study the dry matter production and the nutrient extraction after three consecutive crops, in two soils fertilized with serpentinite rock powder. The experiment was carried in a greenhouse, with three sequential crops, with seven treatments, two soils and four replications. After 40 days of growth, the whole plants were harvested and sent to measure the dry weight and for the chemical analyses. The use of the serpentinite did not increase the total dry matter biomass weight, compared to the fertilized controls. The magnesium extraction was not different between the control treatment with dolomitic limestone and the serpentinite treatment at higher dose for the clayed soil, indicating that the serpentinite was able to supply the magnesium for the plants in the experimental condition, and also a significant dose related effect suggests that the magnesium from the powder has been released. The use of the serpentinite also increased the extraction of other nutrients, like calcium, phosphorus, zinc and iron. |
|
Palavras-Chave: |
Pó de rocha. |
|
Thesagro: |
Fertilizante; Magnésio; Solo. |
|
Thesaurus NAL: |
Urochloa brizantha; Urochloa ruziziensis. |
|
Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
|
Marc: |
LEADER 02126naa a2200265 a 4500
001 2163721
005 2024-04-23
008 2024 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.55905/oelv22n4-068$2DOI
100 1 $aVIANA, J. H. M.
245 $aDry matter production and nutrient extraction by three successive crops fertilized with serpentinite rock as a soil remineralizer.$h[electronic resource]
260 $c2024
520 $aThe incorporation of new sources of fertilizers is important to of the food production security. The use of rock powders still needs investigation to address their agronomic efficiency and the safety for the users and for the consumers. This work aimed to study the dry matter production and the nutrient extraction after three consecutive crops, in two soils fertilized with serpentinite rock powder. The experiment was carried in a greenhouse, with three sequential crops, with seven treatments, two soils and four replications. After 40 days of growth, the whole plants were harvested and sent to measure the dry weight and for the chemical analyses. The use of the serpentinite did not increase the total dry matter biomass weight, compared to the fertilized controls. The magnesium extraction was not different between the control treatment with dolomitic limestone and the serpentinite treatment at higher dose for the clayed soil, indicating that the serpentinite was able to supply the magnesium for the plants in the experimental condition, and also a significant dose related effect suggests that the magnesium from the powder has been released. The use of the serpentinite also increased the extraction of other nutrients, like calcium, phosphorus, zinc and iron.
650 $aUrochloa brizantha
650 $aUrochloa ruziziensis
650 $aFertilizante
650 $aMagnésio
650 $aSolo
653 $aPó de rocha
700 1 $aCOELHO, A. M.
700 1 $aTHOMAZINI, A.
700 1 $aCARVALHO, M. P. F. de
700 1 $aFRANÇA, A. C. F.
700 1 $aCLEMENTE, E. de P.
773 $tObservatorio de La Economia Latinoamerica$gv. 22, n. 4, p. 1-17, 2024.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
